Poly-Prep® Columns, AG® 50W-X8, hydrogen form #7316214

Instructions for Use

1. Shake the column and allow all the resin to settle to the bottom.

2. Remove the column cap to allow the eluant to flow freely.

3. Snap off the seal at the bottom of the column.

4. Monitor the level of liquid in the reservoir,and do not allow the resin to become dry.

5. Rinse the resin with several bed volumes(at least 2 ml) of the sample solvent.Discard rinse.

6. Add sample.

7. Collect effluent.

8. To reduce the flow rate or stop the flow,use a Luer